PHYTOPLANKTON PIGMENT MONITORING ON THE WEST COAST OF CANADA

 

DATA DICTIONARY:

COLUMN

DEFINITION

Cruise Number

The number of the cruise

Date [yyyy-mm-ddThh:mm:ss]

The date the survey was conducted in UTC

Latitude [decimal degrees]

Latitude of the station location

Longitude [decimal degrees]

Longitude of the station location

Pressure [decibar]

Pressure at the sampling depth

HPLC:Chl-c3 [mg/m^3]

Concentration of chlorophyll c3

HPLC:Chlide-a [mg/m^3]

Concentration of chlorophyllide a

HPLC:MgDVP [mg/m^3]

Concentration of Mg-2,4,-divinyl pheoporphyrin a5 monomethyl ester

HPLC:Chl-c2 [mg/m^3]

Concentration of chlorophyll c2

HPLC:Chl-c1 [mg/m^3]

Concentration of chlorophyll c1

HPLC:Me-chlide [mg/m^3]

Concentration of methyl chlorophyllide a

HPLC:Peri [mg/m^3]

Concentration of peridinin

HPLC:Pheide-a [mg/m^3]

Concentration of pheophorbide a

HPLC:But-fuco [mg/m^3]

Concentration of 19’-butanoyloxyfucoxanthin

HPLC:Fuco [mg/m^3]

Concentration of fucoxanthin

HPLC:Neo [mg/m^3]

Concentration of neoxanthin

HPLC:Pras [mg/m^3]

Concentration of prasinoxanthin

HPLC:Viola [mg/m^3]

Concentration of violaxanthin

HPLC:Hex-fuco [mg/m^3]

Concentration of 19’-hexanoyloxyfucoxanthin

HPLC:Diadino [mg/m^3]

Concentration of diadinoxanthin

HPLC:Allo [mg/m^3]

Concentration of alloxanthin

HPLC:Diato [mg/m^3]

Concentration of diatoxanthin

HPLC:Zea [mg/m^3]

Concentration of zeaxanthin

HPLC:Lut [mg/m^3]

Concentration of lutein

HPLC:Chl-b [mg/m^3]

Concentration of chlorophyll b

HPLC:C2mgdg [mg/m^3]

Concentration of chlorophyll c2-monogalactosyldiacylglyceride ester

HPLC:DVChl-a [mg/m^3]

Concentration of divinyl chlorophyll a

HPLC:Chl-a [mg/m^3]

Concentration of chlorophyll a

HPLC:Phe [mg/m^3]

Concentration of pheophytin a

HPLC:B-Car [mg/m^3]

Concentration of β-carotene

HPLC:TChl-a [mg/m^3]

Concentration of total chlorophyll a (is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide)

Flag:HPLC

Flag values have the following significance:

2 = Acceptable Measurement

3 = Questionable Measurement (Probably Good)

4 = Poor Measurement (Probably Bad)

6 = Mean of replicate measurements

Two-digit values indicate two independent flags (e.g. 46 indicates that flag 4 and 6 applies)

 

NOTE: HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Peña, 2014. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value and missing values -99.

 

Nemcek, N. and Peña, M. A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117,  x + 80 p. https://waves-vagues.dfo-mpo.gc.ca/Library/356308.pdf

 

SURVEILLANCE DES PIGMENTS DE PHYTOPLANCTON SUR LA CÔTE OUEST DU CANADA

 

DICTIONNAIRE DE DONNÉES:

COLONNE

DÉFINITION

Numéro de la croisière

Le numéro de la croisière

Date [aaaa-mm-jjThh:mm:ss]

Date à laquelle le relevé a été effectué en TUC

Latitude [degrés décimaux]

Latitude de l’emplacement de la station

Longitude [degrés décimaux]

Longitude de l’emplacement de la station

Pression [décibar]

Pression à la profondeur d’échantillonnage

CLHP:Chl-c3 [mg/m^3]

Concentration de chlorophylle c3

CLHP:Chlide-a [mg/m^3]

Concentration de chlorophyllide a

CLHP:MgDVP [mg/m^3]

Concentration d’ester monométhylique de Mg-2,4-divinyl phéoporphyrine a5

CLHP:Chl-c2 [mg/m^3]

Concentration de chlorophylle c2

CLHP:Chl-c1 [mg/m^3]

Concentration de chlorophylle c1

CLHP:Me-chlide [mg/m^3]

Concentration de methyl chlorophyllide a

CLHP:Péri [mg/m^3]

Concentration de péridinine

CLHP:Phéide-a [mg/m^3]

Concentration de phéophorbide a

CLHP:But-fuco [mg/m^3]

Concentration de 19’-butanoyloxyfucoxanthin

CLHP:Fuco [mg/m^3]

Concentration de fucoxanthine

CLHP:Néo [mg/m^3]

Concentration de néoxanthine

CLHP:Pras [mg/m^3]

Concentration de prasinoxanthine

CLHP:Viola [mg/m^3]

Concentration de violaxanthine

CLHP:Hex-Fuco [mg/m^3]

Concentration de 19’ -hexanoyloxyfucoxanthin

CLHP:Diadino [mg/m^3]

Concentration de diadinoxanthine

CLHP:Allo [mg/m^3]

Concentration d’alloxanthine

CLHP:Diato [mg/m^3]

Concentration de diatoxanthine

CLHP:Zéa [mg/m^3]

Concentration de zéaxanthine

CLHP:Lut [mg/m^3]

Concentration de lutéine

CLHP:Chl-b [mg/m^3]

Concentration de chlorophylle b

CLHP:C2mgdg [mg/m^3]

Concentration d’ester monogalactosyldiacylglycéride de chlorophylle c2

CLHP:DVChl-a [mg/m^3]

Concentration de divinyl chlorophylle a

CLHP:Chl-a [mg/m^3]

Concentration de chlorophylle a

CLHP:Phe [mg/m^3]

Concentration de phéophytine a

CLHP:B-Car [mg/m^3]

Concentration de β-carotène

CLHP:TChl-a [mg/m^3]

Concentration de chlorophylle a totale (la somme de Chl-a, DVchl-a, Chlide-a et Me-chlide)

Indicateurs:CLHP

Les valeurs de l’indicateur ont la signification suivante:
2 = Mesure acceptable
3 = Mesure douteuse (probablement bonne)

4 = Mauvaise mesure (probablement mauvaise)

6 = Moyenne des mesures répétées

Les indicateurs à deux chiffres indiquent deux indicateurs indépendants (par exemple, 46 indique que les indicateurs 4 et 6 s’ appliquent)

 

REMARQUE: Les échantillons pour la CLHP ont été filtrés au moyen de filtres GF/F 47 mm et entreposés à -80 °C avant l’analyse. Ils ont été extraits dans du méthanol à 95 % à -20 °C pendant 24 heures en laboratoire et analysés dans un système de séparation par CLHP WATERS 2695, comme il est décrit en détail dans Nemcek et Pena, 2014. La moyenne des deux échantillons est indiquée. La variabilité est évaluée comme le % du CV (écart-type/moyenne*100) pour les paires en double. Tous les pigments inférieurs à la limite de détection (LD) reçoivent une valeur nulle et les valeurs manquantes -99.

 

Nemcek, N. and Peña, M. A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117, x + 80 p. [en anglais seulement]

https://waves-vagues.dfo-mpo.gc.ca/Library/356308.pdf