PHYTOPLANKTON PIGMENT MONITORING ON THE WEST COAST OF CANADA
DATA DICTIONARY:
COLUMN |
DEFINITION |
Cruise Number |
The number of the cruise |
Date [yyyy-mm-ddThh:mm:ss] |
The date the survey was conducted in UTC |
Latitude [decimal degrees] |
Latitude of the station location |
Longitude [decimal degrees] |
Longitude of the station location |
Pressure [decibar] |
Pressure at the sampling depth |
HPLC:Chl-c3 [mg/m^3] |
Concentration of chlorophyll c3 |
HPLC:Chlide-a [mg/m^3] |
Concentration of chlorophyllide a |
HPLC:MgDVP [mg/m^3] |
Concentration of Mg-2,4,-divinyl pheoporphyrin a5 monomethyl ester |
HPLC:Chl-c2 [mg/m^3] |
Concentration of chlorophyll c2 |
HPLC:Chl-c1 [mg/m^3] |
Concentration of chlorophyll c1 |
HPLC:Me-chlide [mg/m^3] |
Concentration of methyl chlorophyllide a |
HPLC:Peri [mg/m^3] |
Concentration of peridinin |
HPLC:Pheide-a [mg/m^3] |
Concentration of pheophorbide a |
HPLC:But-fuco [mg/m^3] |
Concentration of 19’-butanoyloxyfucoxanthin |
HPLC:Fuco [mg/m^3] |
Concentration of fucoxanthin |
HPLC:Neo [mg/m^3] |
Concentration of neoxanthin |
HPLC:Pras [mg/m^3] |
Concentration of prasinoxanthin |
HPLC:Viola [mg/m^3] |
Concentration of violaxanthin |
HPLC:Hex-fuco [mg/m^3] |
Concentration of 19’-hexanoyloxyfucoxanthin |
HPLC:Diadino [mg/m^3] |
Concentration of diadinoxanthin |
HPLC:Allo [mg/m^3] |
Concentration of alloxanthin |
HPLC:Diato [mg/m^3] |
Concentration of diatoxanthin |
HPLC:Zea [mg/m^3] |
Concentration of zeaxanthin |
HPLC:Lut [mg/m^3] |
Concentration of lutein |
HPLC:Chl-b [mg/m^3] |
Concentration of chlorophyll b |
HPLC:C2mgdg [mg/m^3] |
Concentration of chlorophyll c2-monogalactosyldiacylglyceride ester |
HPLC:DVChl-a [mg/m^3] |
Concentration of divinyl chlorophyll a |
HPLC:Chl-a [mg/m^3] |
Concentration of chlorophyll a |
HPLC:Phe [mg/m^3] |
Concentration of pheophytin a |
HPLC:B-Car [mg/m^3] |
Concentration of β-carotene |
HPLC:TChl-a [mg/m^3] |
Concentration of total chlorophyll a (is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide) |
Flag:HPLC |
Flag values have the following significance: 2 = Acceptable Measurement 3 = Questionable Measurement (Probably Good) 4 = Poor Measurement (Probably Bad) 6 = Mean of replicate measurements Two-digit values indicate two independent flags (e.g. 46 indicates that flag 4 and 6 applies) |
NOTE: HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Peña, 2014. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value and missing values -99.
Nemcek, N. and Peña, M. A. 2014. Institute
of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can.
Tech. Rep. Fish. Aquat. Sci. 3117,
x + 80 p. https://waves-vagues.dfo-mpo.gc.ca/Library/356308.pdf
SURVEILLANCE DES PIGMENTS DE
PHYTOPLANCTON SUR LA CÔTE OUEST DU CANADA
DICTIONNAIRE DE DONNÉES:
COLONNE |
DÉFINITION |
Numéro de la croisière |
Le numéro de la croisière |
Date [aaaa-mm-jjThh:mm:ss] |
Date à laquelle le relevé
a été effectué en TUC |
Latitude [degrés décimaux] |
Latitude de l’emplacement
de la station |
Longitude [degrés
décimaux] |
Longitude de l’emplacement
de la station |
Pression [décibar] |
Pression à la profondeur
d’échantillonnage |
CLHP:Chl-c3 [mg/m^3] |
Concentration de
chlorophylle c3 |
CLHP:Chlide-a [mg/m^3] |
Concentration de chlorophyllide a |
CLHP:MgDVP [mg/m^3] |
Concentration d’ester monométhylique de Mg-2,4-divinyl phéoporphyrine
a5 |
CLHP:Chl-c2 [mg/m^3] |
Concentration de
chlorophylle c2 |
CLHP:Chl-c1 [mg/m^3] |
Concentration de
chlorophylle c1 |
CLHP:Me-chlide [mg/m^3] |
Concentration de methyl chlorophyllide a |
CLHP:Péri [mg/m^3] |
Concentration de péridinine |
CLHP:Phéide-a [mg/m^3] |
Concentration de phéophorbide a |
CLHP:But-fuco [mg/m^3] |
Concentration de
19’-butanoyloxyfucoxanthin |
CLHP:Fuco [mg/m^3] |
Concentration de fucoxanthine |
CLHP:Néo [mg/m^3] |
Concentration de néoxanthine |
CLHP:Pras [mg/m^3] |
Concentration de prasinoxanthine |
CLHP:Viola [mg/m^3] |
Concentration de violaxanthine |
CLHP:Hex-Fuco [mg/m^3] |
Concentration de 19’ -hexanoyloxyfucoxanthin |
CLHP:Diadino [mg/m^3] |
Concentration de diadinoxanthine |
CLHP:Allo [mg/m^3] |
Concentration d’alloxanthine |
CLHP:Diato [mg/m^3] |
Concentration de diatoxanthine |
CLHP:Zéa [mg/m^3] |
Concentration de zéaxanthine |
CLHP:Lut [mg/m^3] |
Concentration de lutéine |
CLHP:Chl-b [mg/m^3] |
Concentration de
chlorophylle b |
CLHP:C2mgdg [mg/m^3] |
Concentration d’ester monogalactosyldiacylglycéride de chlorophylle c2 |
CLHP:DVChl-a [mg/m^3] |
Concentration de divinyl chlorophylle a |
CLHP:Chl-a [mg/m^3] |
Concentration de
chlorophylle a |
CLHP:Phe [mg/m^3] |
Concentration de phéophytine a |
CLHP:B-Car [mg/m^3] |
Concentration de
β-carotène |
CLHP:TChl-a [mg/m^3] |
Concentration de
chlorophylle a totale (la somme de Chl-a, DVchl-a, Chlide-a et Me-chlide) |
Indicateurs:CLHP |
Les valeurs de
l’indicateur ont la signification suivante: 4 = Mauvaise mesure (probablement
mauvaise) 6 = Moyenne des mesures
répétées Les indicateurs à
deux chiffres indiquent deux indicateurs indépendants
(par exemple, 46 indique que les indicateurs 4 et 6 s’ appliquent) |
REMARQUE: Les échantillons
pour la CLHP ont été filtrés au moyen de filtres GF/F 47 mm et entreposés à -80
°C avant l’analyse. Ils ont été extraits dans du méthanol à 95 % à -20 °C
pendant 24 heures en laboratoire et analysés dans un système de séparation par
CLHP WATERS 2695, comme il est décrit en détail dans Nemcek et Pena, 2014. La
moyenne des deux échantillons est indiquée. La variabilité est évaluée comme le
% du CV (écart-type/moyenne*100) pour les paires en double. Tous les pigments
inférieurs à la limite de détection (LD) reçoivent une valeur nulle et les valeurs
manquantes -99.
Nemcek, N. and Peña, M. A. 2014. Institute of Ocean Sciences
Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish.
Aquat. Sci. 3117, x + 80 p.
[en anglais seulement]
https://waves-vagues.dfo-mpo.gc.ca/Library/356308.pdf